ACCESSION NO: 96-97-1641 TITLE: Deadly Enigma AUTHOR: BEARDSLEY, TIM JOURNAL: Scientific American CITATION: December, 1996, 275: 16, 18. YEAR: 1996 PUB TYPE: Article IDENTIFIERS: PROTEINS; MAD COW DISEASE; TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES; CREUTZFELDT-JAKOB DISEASE; PRION PROTEIN; BRAIN DISEASES; DISEASE TRANSMISSION ABSTRACT: An abnormal form of a protein present in all mammals causes at least 15 different lethal brain diseases affecting a wide variety of animals. Some mysterious agent that resists standard chemical disinfection and high temperatures can transmit these diseases--such as scrapie in sheep, mad cow disease in cattle, and Creutzfeldt-Jakob disease in humans-- between individuals and even between species. It is unknown how the agent spreads under natural conditions and how it destroys brain tissue. The diseases are called transmissible spongiform encephalopathies (TSEs) because of the characteristic spongelike appearance of brain tissue. Mad cow disease has turned into a national crisis in Great Britain, and there is fear that the disease, which causes animals to become nervous and develop an unsteady gait, will spread to people. British researchers have identified 14 unusual cases of Creutzfeldt-Jakob disease in young people they suspect were a human manifestation of mad cow disease, and new studies appear to strengthen that conclusion. Researchers now theorize that a ubiquitous mammalian protein called prion protein can, rarely, refold itself into a toxic form that then spreads conversion of more healthy protein in a runaway process. Some mutant forms of the protein are more likely to convert spontaneously than others, accounting for the rare sporadic cases of TSE in humans. TSEs are both inherited and transmittable, and unlike any other known diseases, the pathogen lacks DNA and RNA. A test for TSEs in humans and in a few animals was announced in September 1996, but so far it seems to perform well only when clear symptoms of illness have already developed.